Signalling

Part:BBa_K2249000:Design

Designed by: Maxwell Saal   Group: iGEM17_CU-Boulder   (2017-10-27)


(EutC1-19)-FusionRed-LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 583
    Illegal BsaI.rc site found at 682


Design Notes

For best visualization the cells should be grown to mid-log phase and then transferred to 20-27C growth conditions for several hours for good balance of protein expression.


Source

Ordered from AddGene by another lab at CU and gifted to us. EutC and LVA degradation tags added by Gibson assembly primers.

References

Shemiakina I.I. et al. A monomeric red fluorescent protein with low cytotoxicity. Nat. Commun. 3:1204 doi: 10.1038/ncomms2208 (2012).